De Novo Metagenomic Analysis of Microbial Community Contributing in Lignocellulose Degradation in Humus Samples Harvested from Cuc Phuong Tropical Forest in Vietnam

We aimed to investigate the microbial diversity, mine lignocellulose-degrading enzymes/proteins, and analyze domain structures of mined enzymes/proteins in humus samples collected from Cuc Phuong National Park, Vietnam. Using a high-throughput Illumina sequencer, 52 Gbs DNA were assembled 2,611,883 contigs, which 4,104,872 open reading frames (ORFs) identified. Among total microbiome analyzed, bacteria occupied 99.69%; five ubiquitous bacterial phyla included Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutes, Acidobacteria, accounted for 92.59%. Proteobacteria (75.68%), most dominant, was 5.77 folds higher than second abundant phylum Bacteroidetes (13.11%). Considering involved lignocellulose degradation, 22,226 ORFs obtained annotation analysis using KEGG database. The estimated ratio Proteobacteria/Bacteroidetes approximately 1:1 pretreatment hemicellulases groups 2.4:1 cellulases. Furthermore, revealed their diversity enzymes. CE PL two main families pretreatment; GH1 GH3-FN3 highest domains cellulase group, whereas GH2 GH43 represented hemicellulase group. These results validate that natural tropical forest soil could be considered as an important source explore novel degradation lignocellulose.